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c4ii hpv18  (ATCC)


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    Structured Review

    ATCC c4ii hpv18
    ( A and B ) Expression of lnc-FANCI-2 (red) in HPV16 + cervical squamous cell carcinoma tissues was examined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). The corresponding regions ( A ) in H&E staining are shown for the adjacent region from the tumor nest and histotype. ( C ) Human foreskin keratinocyte (HFK)-derived raft cultures without (HFK) or with HPV16 or <t>HPV18</t> infections (HFK-HPV16 or HFK-HPV18) were examined at day 10 for lnc-FANCI-2 RNA by RNAscope RNA-ISH analysis. Scale bars: 25 μm in the original figures and 10 μm in the zoomed insets.
    C4ii Hpv18, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c4ii+hpv18/C-4+II/pmc12396819-1-7-12
    Average 94 stars, based on 94 article reviews
    c4ii hpv18 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells"

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells

    Journal: eLife

    doi: 10.7554/eLife.102681

    ( A and B ) Expression of lnc-FANCI-2 (red) in HPV16 + cervical squamous cell carcinoma tissues was examined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). The corresponding regions ( A ) in H&E staining are shown for the adjacent region from the tumor nest and histotype. ( C ) Human foreskin keratinocyte (HFK)-derived raft cultures without (HFK) or with HPV16 or HPV18 infections (HFK-HPV16 or HFK-HPV18) were examined at day 10 for lnc-FANCI-2 RNA by RNAscope RNA-ISH analysis. Scale bars: 25 μm in the original figures and 10 μm in the zoomed insets.
    Figure Legend Snippet: ( A and B ) Expression of lnc-FANCI-2 (red) in HPV16 + cervical squamous cell carcinoma tissues was examined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). The corresponding regions ( A ) in H&E staining are shown for the adjacent region from the tumor nest and histotype. ( C ) Human foreskin keratinocyte (HFK)-derived raft cultures without (HFK) or with HPV16 or HPV18 infections (HFK-HPV16 or HFK-HPV18) were examined at day 10 for lnc-FANCI-2 RNA by RNAscope RNA-ISH analysis. Scale bars: 25 μm in the original figures and 10 μm in the zoomed insets.

    Techniques Used: Expressing, RNAscope, RNA In Situ Hybridization, Staining, Derivative Assay

    ( A ) RT-qPCR detection of lnc-FANCI-2 in HPV16 + cervical cancer cell lines SiHa, CaSki, and W12 20861 (integrated HPV16) and 20863 (episomal HPV16), HPV18 + cervical cancer cell lines HeLa and C4II, and HPV - cell lines C33A (cervical cancer cells with mutations of p53 and pRb), HCT116 (colorectal cancer cells), BCBL-1 (body cavity B lymphoma cells), HEK293 (Ad5 E1/E2-immortalized human kidney cells), and HaCaT (spontaneously immortalized human epidermal cells) in triplicates. ( B ) HeLa cells express no lnc-FANCI-2 when compared with C33A cells by northern blot. ( C ) lnc-FANCI-2 is mainly cytoplasmic in CaSki but nuclear in SiHa and C33A cells. Cytoplasmic and nuclear fractionation efficiency was blotted for nuclear SRSF3 (serine- and arginine-rich splicing factor 3) and cytoplasmic GAPDH. Total fractionated cytoplasmic and nuclear RNAs were quantified for lnc-FANCI-2 by RT-qPCR in triplicates, with GAPDH RNA serving as an internal control for RNA fractionation efficiency. ( D ) Subcellular lnc-FANCI-2 (red) localization in CaSki, SiHa, and C33A cells determined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). Scale bars: 25 μm in the top and 10 μm in the zoom. Figure 2—source data 1. Northern blot and western blot data for . Figure 2—source data 2. Northern blot and western blot data for .
    Figure Legend Snippet: ( A ) RT-qPCR detection of lnc-FANCI-2 in HPV16 + cervical cancer cell lines SiHa, CaSki, and W12 20861 (integrated HPV16) and 20863 (episomal HPV16), HPV18 + cervical cancer cell lines HeLa and C4II, and HPV - cell lines C33A (cervical cancer cells with mutations of p53 and pRb), HCT116 (colorectal cancer cells), BCBL-1 (body cavity B lymphoma cells), HEK293 (Ad5 E1/E2-immortalized human kidney cells), and HaCaT (spontaneously immortalized human epidermal cells) in triplicates. ( B ) HeLa cells express no lnc-FANCI-2 when compared with C33A cells by northern blot. ( C ) lnc-FANCI-2 is mainly cytoplasmic in CaSki but nuclear in SiHa and C33A cells. Cytoplasmic and nuclear fractionation efficiency was blotted for nuclear SRSF3 (serine- and arginine-rich splicing factor 3) and cytoplasmic GAPDH. Total fractionated cytoplasmic and nuclear RNAs were quantified for lnc-FANCI-2 by RT-qPCR in triplicates, with GAPDH RNA serving as an internal control for RNA fractionation efficiency. ( D ) Subcellular lnc-FANCI-2 (red) localization in CaSki, SiHa, and C33A cells determined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). Scale bars: 25 μm in the top and 10 μm in the zoom. Figure 2—source data 1. Northern blot and western blot data for . Figure 2—source data 2. Northern blot and western blot data for .

    Techniques Used: Quantitative RT-PCR, Northern Blot, Fractionation, Control, RNAscope, RNA In Situ Hybridization, Staining, Western Blot

    Related Articles

    Expressing:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    RNAscope:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    RNA In Situ Hybridization:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Staining:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Derivative Assay:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Quantitative RT-PCR:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Northern Blot:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Fractionation:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Control:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Western Blot:

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells
    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.



    Similar Products

    c4ii hpv18  (ATCC)
    94
    ATCC c4ii hpv18
    ( A and B ) Expression of lnc-FANCI-2 (red) in HPV16 + cervical squamous cell carcinoma tissues was examined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). The corresponding regions ( A ) in H&E staining are shown for the adjacent region from the tumor nest and histotype. ( C ) Human foreskin keratinocyte (HFK)-derived raft cultures without (HFK) or with HPV16 or <t>HPV18</t> infections (HFK-HPV16 or HFK-HPV18) were examined at day 10 for lnc-FANCI-2 RNA by RNAscope RNA-ISH analysis. Scale bars: 25 μm in the original figures and 10 μm in the zoomed insets.
    C4ii Hpv18, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c4ii+hpv18/C-4+II/pmc12396819-1-7-12
    Average 94 stars, based on 1 article reviews
    c4ii hpv18 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A and B ) Expression of lnc-FANCI-2 (red) in HPV16 + cervical squamous cell carcinoma tissues was examined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). The corresponding regions ( A ) in H&E staining are shown for the adjacent region from the tumor nest and histotype. ( C ) Human foreskin keratinocyte (HFK)-derived raft cultures without (HFK) or with HPV16 or HPV18 infections (HFK-HPV16 or HFK-HPV18) were examined at day 10 for lnc-FANCI-2 RNA by RNAscope RNA-ISH analysis. Scale bars: 25 μm in the original figures and 10 μm in the zoomed insets.

    Journal: eLife

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells

    doi: 10.7554/eLife.102681

    Figure Lengend Snippet: ( A and B ) Expression of lnc-FANCI-2 (red) in HPV16 + cervical squamous cell carcinoma tissues was examined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). The corresponding regions ( A ) in H&E staining are shown for the adjacent region from the tumor nest and histotype. ( C ) Human foreskin keratinocyte (HFK)-derived raft cultures without (HFK) or with HPV16 or HPV18 infections (HFK-HPV16 or HFK-HPV18) were examined at day 10 for lnc-FANCI-2 RNA by RNAscope RNA-ISH analysis. Scale bars: 25 μm in the original figures and 10 μm in the zoomed insets.

    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Techniques: Expressing, RNAscope, RNA In Situ Hybridization, Staining, Derivative Assay

    ( A ) RT-qPCR detection of lnc-FANCI-2 in HPV16 + cervical cancer cell lines SiHa, CaSki, and W12 20861 (integrated HPV16) and 20863 (episomal HPV16), HPV18 + cervical cancer cell lines HeLa and C4II, and HPV - cell lines C33A (cervical cancer cells with mutations of p53 and pRb), HCT116 (colorectal cancer cells), BCBL-1 (body cavity B lymphoma cells), HEK293 (Ad5 E1/E2-immortalized human kidney cells), and HaCaT (spontaneously immortalized human epidermal cells) in triplicates. ( B ) HeLa cells express no lnc-FANCI-2 when compared with C33A cells by northern blot. ( C ) lnc-FANCI-2 is mainly cytoplasmic in CaSki but nuclear in SiHa and C33A cells. Cytoplasmic and nuclear fractionation efficiency was blotted for nuclear SRSF3 (serine- and arginine-rich splicing factor 3) and cytoplasmic GAPDH. Total fractionated cytoplasmic and nuclear RNAs were quantified for lnc-FANCI-2 by RT-qPCR in triplicates, with GAPDH RNA serving as an internal control for RNA fractionation efficiency. ( D ) Subcellular lnc-FANCI-2 (red) localization in CaSki, SiHa, and C33A cells determined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). Scale bars: 25 μm in the top and 10 μm in the zoom. Figure 2—source data 1. Northern blot and western blot data for . Figure 2—source data 2. Northern blot and western blot data for .

    Journal: eLife

    Article Title: The long noncoding RNA lnc-FANCI-2 intrinsically restricts RAS signaling in human papillomavirus type 16-infected cervical cancer cells

    doi: 10.7554/eLife.102681

    Figure Lengend Snippet: ( A ) RT-qPCR detection of lnc-FANCI-2 in HPV16 + cervical cancer cell lines SiHa, CaSki, and W12 20861 (integrated HPV16) and 20863 (episomal HPV16), HPV18 + cervical cancer cell lines HeLa and C4II, and HPV - cell lines C33A (cervical cancer cells with mutations of p53 and pRb), HCT116 (colorectal cancer cells), BCBL-1 (body cavity B lymphoma cells), HEK293 (Ad5 E1/E2-immortalized human kidney cells), and HaCaT (spontaneously immortalized human epidermal cells) in triplicates. ( B ) HeLa cells express no lnc-FANCI-2 when compared with C33A cells by northern blot. ( C ) lnc-FANCI-2 is mainly cytoplasmic in CaSki but nuclear in SiHa and C33A cells. Cytoplasmic and nuclear fractionation efficiency was blotted for nuclear SRSF3 (serine- and arginine-rich splicing factor 3) and cytoplasmic GAPDH. Total fractionated cytoplasmic and nuclear RNAs were quantified for lnc-FANCI-2 by RT-qPCR in triplicates, with GAPDH RNA serving as an internal control for RNA fractionation efficiency. ( D ) Subcellular lnc-FANCI-2 (red) localization in CaSki, SiHa, and C33A cells determined by RNAscope RNA in situ hybridization (RNA-ISH) analysis. Nuclei were stained with DAPI (blue). Scale bars: 25 μm in the top and 10 μm in the zoom. Figure 2—source data 1. Northern blot and western blot data for . Figure 2—source data 2. Northern blot and western blot data for .

    Article Snippet: Cell line ( Homo sapiens ) , C4II (HPV18 + ) , ATCC , CRL-1595 , Cervical cancer cell line.

    Techniques: Quantitative RT-PCR, Northern Blot, Fractionation, Control, RNAscope, RNA In Situ Hybridization, Staining, Western Blot